The Proteomics Shared Resource (PSR) provides services for discovery and targeted proteomics using tissues (e.g. fresh frozen and formalin fixed), cells, and biological fluids (e.g. plasma/serum, cerebrospinal fluid and urine)1. The PSR performs small (≤ 20 samples) and large-scale studies with ~100 clinical samples1,2. Validated proteomics workflows for labeled (tandem-mass-tag) 2,3 and label-free quantification are offered. The PSR provides quantitative proteomics data analysis with “hands-on” assistance and the customized software Proteo-Q.
Deep scale proteomics (10,000 to 13,000 genes), phosphorylation (37,000 to 100,000 phosphosites), acetylation (~2000 sites), and ubiquitination (~2000 sites) are achieved using validated methods4,5. For additional workflow examples, contact PSR.
Absolute quantification (copies/cell) is performed using synthetic surrogate peptides with stable isotope dilution mass spectrometry.
Kinome (304 kinases) and metabolic enzyme (67 enzymes) stable isotope peptide libraries are available with standard assays developed under Clinical Proteomics Tumor Analysis Consortium (CPTAC) guidelines.
Assistance with experimental design (statistics-based), data processing, analysis and interpretation by the Director and Co-directors is available both before and after experiments are performed.
*Listed prices include sample preparation and mass spectrometer data acquisition. Data analysis of raw mass spectrometer machine data may be procured from the Proteomics Data Analysis Core in the Division of Endocrinology, Metabolism and Lipid Research (contact Dr. Tim Zhang at zhang.qiang@wustl.edu).
| BILLING ITEM | WASHU PRICING | EXTERNAL PRICING |
| Advanced Protein Identification (includes ultrasonic solubilization, S-trap digestion, and 1 hr RAW timsTOF machine data) | $667.00 | $800.40 |
Advanced Protein Identification for Quantitation | ||
Label-free quantitation (includes ultrasonic solubilization, S-trap digestion, and 1 hr RAW timsTOF machine data) | $667.00 | $800.40 |
Tandem Mass Tag Quantitation – TMT10 (includes reference pool, ultrasonic solubilization, peptide generation, TMT peptide labeling, and 4.4 hr RAW Q-Exactive machine data) | $680.38 | $816.45 |
Phosphoproteomics (100 ug peptide input, ~10,000 phosphosites) or Nano-scale Phosphoproteomics (10 ug peptide input, ~3,000 phosphosites) | $918.00 | $1,101.60 |
Deep Scale Global Proteomics, Phosphoproteomics, and Acetylomics (contact Dr. Robert Sprung for pricing at robert.sprung@wustl.edu) | ||
Acetylomics (PTMScan kit, 400 ug peptide input, ~2,000 acetylation sites) | $809.00 | $970.80 |
Ubiquitinomics (PTMScan kit, 400 ug peptide input, ~2,000 ubiquitin sites) | $809.00 | $970.80 |
Proximity Labeling Proteomics | $263.00 | $315.60 |
Proteomics Analysis of Immunoprecipitation Samples | $506.00 | $607.20 |
Note simultaneous sample preparation of 5-9 samples will receive a 10% discount, simultaneous preparation of 10 or more samples will receive a 15% discount.
*Data analysis of RAW mass spectrometer machine data may be procured from the Proteomics Data Analysis Core in the Division of Endocrinology, Metabolism and Lipid Research (contact Dr. Tim Zhang at zhang.qiang@wustl.edu).
For a complete list of prices and services, please contact Dr. Robert Sprung for pricing at robert.sprung@wustl.edu.
(1) Isaacs AM, Morton SU, Movassagh M, Zhang Q, Hehnly C, Zhang L, Morales DM, Sinnar SA, Ericson JE, Mbabazi-Kabachelor E, Ssenyonga P, Onen J, Mulondo R, Hornig M, Warf BC, Broach JR, Townsend RR, Limbrick DD Jr, Paulson JN, Schiff SJ. (2021) Immune activation during Paenibacillus brain infection in African infants with frequent cytomegalovirus co-infection. iScience. 24(4):102351. PMID: 33912816
(2) Kramer MH, Zhang Q, Sprung R, Day RB, Erdmann-Gilmore P, Li Y, Xu Z, Helton NM, George DR, Mi Y, Westervelt P, Payton JE, Ramakrishnan SM, Miller CA, Link DC, DiPersio JF, Walter MJ, Townsend RR, Ley TJ. (2022) Proteomic and phosphoproteomic landscapes of acute myeloid leukemia. Blood. 140(13):1533-1548. PMID: 35895896
(3) Sprung RW, Zhang Q, Kramer MH, Christopher MC, Erdmann-Gilmore P, Mi Y, Malone JP, Ley TJ, Townsend RR. (2024) Stabilizing the Proteomes of Acute Myeloid Leukemia Cells: Implications for Cancer Proteomics. Mol Cell Proteomics. 23(2):100716. PMID: 38219859
(4) Mertins, P., Qiao, J.W., Patel, J., Udeshi, N.D., Clauser, K.R., Mani, D.R., Burgess, M.W., Gillette, M.A., Jaffe, J.D., and Carr, S.A. (2013). Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-637. PMID: 23749302
(5) Mertins, P., Tang, L.C., Krug, K., Clark, D.J., Gritsenko, M.A., Chen, L., Clauser, K.R., Clauss, T.R., Shah, P., Gillette, M.A., et al. (2018). Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography-mass spectrometry. Nat Protoc 13, 1632-1661. PMID: 29988108
R. Reid Townsend, MD, PhD
Director
rtownsend@wustl.edu
Qiang (Tim) Zhang, PhD
Co-Director
zhang.qiang@wustl.edu
Robert Sprung, PhD
Co-Director
robert.sprung@wustl.edu
Petra Erdmann-Gilmore
Lab Manager
pegilmore@wustl.edu