On December 16, Jeremiah Stitham, MD, PhD and colleagues, had their research titled, “Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence,” published in “STAR Protocols.”
The study provides a “detailed protocol for the evaluation of diet-induced mTORC1 signaling in plaque macrophages in atherosclerosis-prone apolipoprotein E (ApoE) knockout (KO) mice.” By encompassing flow cytometry and immunofluorescence analysis of atherosclerotic macrophages, it allows for better understanding of the atherogenic potential of a variety of mTORC1 modulators.