On April 20, Clair Crewe, PhD and Jessica M. Sabio had their protocol titled “Isolation of Adipose Tissue Extracellular Vesicles,” published in the book series “Thermogenic Fat: Methods in Molecular Biology.”
Between cells in tissue and circulation, you’ll find extracellular vesicles (EV). Which are produced by adipocytes and other adipose tissue (AT) cells and send signals between cells in the tissue and distal organs.
Due to the unique biophysical properties of AT, an optimized EV isolation protocol is required to ensure that EV isolate is uncontaminated. Throughout their work, Crewe and Sabio discuss a protocol that can be used “to isolate and characterize the total, heterogeneous population of EVs from the AT.”
Sabio, J.M., Crewe, C. (2023). Isolation of Adipose Tissue Extracellular Vesicles. In: Lodhi, I.J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 2662. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3167-6_19